Description
EV Isolation
- Description
- Structure & Composition
- Features
- Protocol
- Exosome & Nanoparticles Isolation
- Specification
- Resource
Nanocon
The Nanocon is a single-use device designed for rapid processing of aqueous biological solutions at low centrifugal force (2000G).
Compatible with swing bucket centrifuges only.
The Nanocon device can concentrate and purify a 40ml solution to 4ml in one hour.
- Exosome, virus or recombinant gene isolation and concentration
- New TFF (Tangential Flow Filtration) filtration device
- Effectively filter using the combination of centrifugal force and TFF
- Recover high yield of nano particles larger than 30nm
- Using high porosity and special surface treated membrane
- Running volume to a maximum of 40ml
- Concentration of sample greater than 10 times
- Effectively eliminates impurities smaller than 30nm
- Conventional lab centrifuge can be used
- Low centrifugal force of approx. 2000G
- Nanocon made to fit 250ml centrifuge bottle
Structure & Composition
Features
- Special surface treated membrane
- Uniform pore size of 30nm
- High porosity polymer membrane
Protocol
TFF [Tangential Flow Filtration]
- Large particles are pulled in the direction of centrifugal force
- The fluid flow is in the tangential direction of centrifugal force
[TFF: Tangential Flow Filtration] - Small impurities will be eliminated with flow
- Large particles do not block the filter surface and are effectively filtered
Exosome & Nanoparticles Isolation
- Isolation and concentration of exosome in cell culture supernatant, urine, plant-derived fluid, Ascites, Bronchial washing fluid, etc.
- No chemicals are used
- Intact exosome can be obtained after filtration
- Isolation and concentration of viruses larger than 30nm
- Isolation and concentration of recombinant genes larger than 30nm
| CCS | |
| Sample Volume | 80ml → 4ml |
| 20X | |
| Time | 95 min |
| BCA (ug/ml) | 0.85 |
| NTA (particles/ml, CCS) | 1.45X108 |
| Total particles (/80ml) | 1.16X1010 |
| Urine | |
| Sample Volume | 140ml → 4ml |
| 35X | |
| Time | 120 min |
| BCA (ug/ml) | 4.37 |
| NTA (particles/ml, Urine) | 1.6X108 |
| Total particles (/140ml) | 2.24X1010 |
Specification
Model no. NNC-30
| Maximum sample volume | 40 ml |
| Final concentrate volume | 4 ml (UP) / 1 ml (LV) |
| Maximum centrifugal force | 2000 x g (recommend) |
| Active membrane surface area | 13.7cm2 |
| Items | Cat. no. | Specification |
|---|---|---|
| Nanocon (upper) | NNC-30-UP (10ea/1box) | 30nm polymer membrane, special treated surface Concentrate up to 4ml |
| Nanocon (lower) | NNC-30-LV (10ea/1box) | 30nm polymer membrane, special treated surface Concentrate up to 1ml |
| Centrifuge for Nanocon | NL-1 | Max 5000G, 4x adaptor for Nanocon |
NNC-30
Dedicated centrifuge for Nanocon